Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.
Identifieur interne : 002F77 ( Main/Exploration ); précédent : 002F76; suivant : 002F78Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.
Auteurs : Timothy Othman [Canada] ; Christopher J D. Sinclair ; Norman Haughey ; Jonathan D. Geiger ; Fiona E. ParkinsonSource :
- Neurochemical research [ 0364-3190 ] ; 2002.
English descriptors
- KwdEn :
- Adenosine (metabolism), Adenosine Triphosphate (metabolism), Alkaloids, Animals, Astrocytes (drug effects), Astrocytes (metabolism), Benzophenanthridines, Biological Transport (drug effects), Cells, Cultured, Cerebral Cortex (drug effects), Cerebral Cortex (embryology), Cerebral Cortex (metabolism), Embryo, Mammalian, Ethanol (pharmacology), Glutamic Acid (metabolism), Kinetics, Phenanthridines (pharmacology), Protein Kinase C (metabolism), Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Tetradecanoylphorbol Acetate (pharmacology).
- MESH :
- chemical , metabolism : Adenosine, Adenosine Triphosphate, Glutamic Acid, Protein Kinase C.
- chemical , pharmacology : Ethanol, Phenanthridines, Tetradecanoylphorbol Acetate.
- chemical : Alkaloids, Benzophenanthridines.
- drug effects : Astrocytes, Biological Transport, Cerebral Cortex.
- embryology : Cerebral Cortex.
- metabolism : Astrocytes, Cerebral Cortex.
- Animals, Cells, Cultured, Embryo, Mammalian, Kinetics, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Glutamate is the primary excitatory neurotransmitter in brain. By stimulating neuronal activity, glutamate increases cellular energy utilization, enhances ATP hydrolysis and promotes the formation of adenosine. Adenosine has receptor-mediated effects that reduce or oppose the excitatory effects of glutamate. As a possible mechanism for ethanol's ability to inhibit excitatory effects of glutamate and enhance inhibitory effects of adenosine, we tested the hypothesis that ethanol promotes [3H]glutamate uptake and inhibits [3H]adenosine uptake. Using primary cultures of rat astrocytes, we found that acute treatment with ethanol (50 mM, 30 min) inhibited [3H]glutamate uptake and reduced protein kinase C (PKC)-induced stimulation of [3H]glutamate uptake. Prolonged treatment (50 mM, 3 day) with ethanol, however, increased both [3H]glutamate uptake and PKC activity. Contrary to other cell types, neither acute or chronic ethanol exposure affected [3H]adenosine uptake in astrocytes. These data indicate that in rat cortical astrocytes ethanol affects [3H]glutamate uptake but not [3H]adenosine uptake by affecting PKC modulation of transporter activity.
PubMed: 11958530
Affiliations:
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Sinclair</name></author><author><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name></author><author><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name></author><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11958530</idno><idno type="pmid">11958530</idno><idno type="wicri:Area/PubMed/Corpus">001521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001521</idno><idno type="wicri:Area/PubMed/Curation">001521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001521</idno><idno type="wicri:Area/PubMed/Checkpoint">001521</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001521</idno><idno type="wicri:Area/Ncbi/Merge">000217</idno><idno type="wicri:Area/Ncbi/Curation">000217</idno><idno type="wicri:Area/Ncbi/Checkpoint">000217</idno><idno type="wicri:doubleKey">0364-3190:2002:Othman T:ethanol:alters:glutamate</idno><idno type="wicri:Area/Main/Merge">003407</idno><idno type="wicri:Area/Main/Curation">002F77</idno><idno type="wicri:Area/Main/Exploration">002F77</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.</title><author><name sortKey="Othman, Timothy" sort="Othman, Timothy" uniqKey="Othman T" first="Timothy" last="Othman">Timothy Othman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Sinclair, Christopher J D" sort="Sinclair, Christopher J D" uniqKey="Sinclair C" first="Christopher J D" last="Sinclair">Christopher J D. Sinclair</name></author><author><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name></author><author><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name></author><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name></author></analytic><series><title level="j">Neurochemical research</title><idno type="ISSN">0364-3190</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine (metabolism)</term><term>Adenosine Triphosphate (metabolism)</term><term>Alkaloids</term><term>Animals</term><term>Astrocytes (drug effects)</term><term>Astrocytes (metabolism)</term><term>Benzophenanthridines</term><term>Biological Transport (drug effects)</term><term>Cells, Cultured</term><term>Cerebral Cortex (drug effects)</term><term>Cerebral Cortex (embryology)</term><term>Cerebral Cortex (metabolism)</term><term>Embryo, Mammalian</term><term>Ethanol (pharmacology)</term><term>Glutamic Acid (metabolism)</term><term>Kinetics</term><term>Phenanthridines (pharmacology)</term><term>Protein Kinase C (metabolism)</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Tetradecanoylphorbol Acetate (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine</term><term>Adenosine Triphosphate</term><term>Glutamic Acid</term><term>Protein Kinase C</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Ethanol</term><term>Phenanthridines</term><term>Tetradecanoylphorbol Acetate</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Alkaloids</term><term>Benzophenanthridines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Astrocytes</term><term>Biological Transport</term><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Astrocytes</term><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Embryo, Mammalian</term><term>Kinetics</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glutamate is the primary excitatory neurotransmitter in brain. By stimulating neuronal activity, glutamate increases cellular energy utilization, enhances ATP hydrolysis and promotes the formation of adenosine. Adenosine has receptor-mediated effects that reduce or oppose the excitatory effects of glutamate. As a possible mechanism for ethanol's ability to inhibit excitatory effects of glutamate and enhance inhibitory effects of adenosine, we tested the hypothesis that ethanol promotes [3H]glutamate uptake and inhibits [3H]adenosine uptake. Using primary cultures of rat astrocytes, we found that acute treatment with ethanol (50 mM, 30 min) inhibited [3H]glutamate uptake and reduced protein kinase C (PKC)-induced stimulation of [3H]glutamate uptake. Prolonged treatment (50 mM, 3 day) with ethanol, however, increased both [3H]glutamate uptake and PKC activity. Contrary to other cell types, neither acute or chronic ethanol exposure affected [3H]adenosine uptake in astrocytes. These data indicate that in rat cortical astrocytes ethanol affects [3H]glutamate uptake but not [3H]adenosine uptake by affecting PKC modulation of transporter activity.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><noCountry><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name><name sortKey="Sinclair, Christopher J D" sort="Sinclair, Christopher J D" uniqKey="Sinclair C" first="Christopher J D" last="Sinclair">Christopher J D. Sinclair</name></noCountry><country name="Canada"><region name="Manitoba"><name sortKey="Othman, Timothy" sort="Othman, Timothy" uniqKey="Othman T" first="Timothy" last="Othman">Timothy Othman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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